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Utilizing the novel Bridge Capture™ approach, Geno1® empowers a new generation of kitted oncology sequencing tests.
Geno1® excels in simplicity, scalability, and cost, driving innovation in liquid biopsy field. Geno1® is agnostic to NGS platforms, ensuring broad applicability. The technology is offered through out-licensing, providing a valuable opportunity for partnership and growth.
It’s a turnkey technology that’s unlocking the future of distributed kits on a global scale.
The core of the Geno1® chemistry is the Probe Library, which is based on proprietary Probe Design. The Probe Library is a stock of pre-designed probes that defines the content of the assay. The chemistry also includes bulk enzymes and reagents needed in Geno1®.
Specific targets for the panel to be assembled
Geno1® workflow is a few-step patented laboratory process that leads to targeted NGS libraries for sequencing, it can be fully automated with robotics – and has a prep-time of less than 5 hours.
Sequencing libraries based on the defined targets for the panel, ready for sequencing
After sequencing the data is processed by our proprietary algorithms. This data can further be used with other data sources for creating clinical interpretations and reports, by our customers´ own bioinformatics pipeline. A suitable cloud-based service is used to deploy this part.
Patient specific mutation profiles, based on the content of the panel and findings from the samples as VCF file
Geno1® marks a significant advancement in NGS technology by integrating the strengths of amplicon and hybrid capture methods, enhancing scalability, sensitivity, and workflow efficiency. With its UMI-based protocol, Geno1® offers a leap in simplicity and scalability, achieving high evenness and sensitivity for rare variants. This translates to a streamlined, cost-efficient process, surpassing the multiplexing and sensitivity constraints of traditional amplicon techniques.
Moreover, Geno1® streamlines the complexity typically associated with target enrichment, delivering a swift and simplified workflow. By focusing on pre-selected loci, Geno1® ensures high target evenness, enabling the detection of rare variants at moderate sequencing depths. It presents a cost-effective and efficient alternative to target enrichment’s extensive workflows, high sequencing demands, and complicated data analysis.
In short, Geno1® combines the strengths of both amplicon and hybrid capture technologies, offering excellent sensitivity and scalability, with a user-friendly protocol that significantly reduces cost and complexity in NGS workflows.
Successful targeting of a specific sequence is achieved by the simultaneous binding of two probes, held together by a bridge oligo. The requirement for simultaneous probe binding to their respective target sequences enhances the specificity of the bridge capture process. UMIs included in the probes enable accurate identification of unique binding events.
In the gap fill step, the DNA polymerase extends the probes using the target DNA as a template, followed by ligation to form a continuous circular structure, with the bridge oligo intact.
The circular construct is linearly amplified by RCA to generate multiple copies of the circular probe, ensuring non-biased amplification that accurately retains molecular ratios. After RCA, the single-stranded concatemers formed are digested by a restriction enzyme, resulting in multiple copies of targeted amplicons.
Limited Cycle PCR attaches sequencing platform-specific adapters to the digestion products, minimizing the exponential amplification bias.
This process is collectively known as Bridge Capture™.
Genomill has established a strong IPR portfolio for Geno1® and Bridge Capture™. The Geno003 stands as a key patent in our laboratory methods.
Geno001
Foundational patent
US-2023340564
EP-3673081
CA-3072650
CN-111032881
JP-7074978
Geno002
Dirty samples
US-11898202
EP-4060052
CA-3149035
CN-115109846
JP-2022145605
KR-20220130591
TW-202302861
Geno003
Improved workflow
US-11486003
EP-4060053
CA-3149025
CN-115109842
JP-2022145606
KR-20220130592
Geno004
Alternative workflow
EP-4215619
Geno005
Improved sample indexing and barcodes
US-2024068022
EP-4332238
CA-3208897
CN-117625764
JP-2024035109
KR-20240032630
Geno006
Allelic boosting for rare variants
US-2024068010
EP-4332235
CA-3208896
CN-117625763
JP-2024035110
KR-20240032631